| 产品名称: |
Recombinant Human PDGF-BB |
| 产品形貌: |
Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed. |
| 卵白编号: |
P01127 |
| 分子量: |
12.42 KDa |
| 表观分子量: |
14 KDa, reducing conditions |
| 产品纯度: |
Greater than 98% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.02 ng/?g (0.2 EU/?g) as determined by LAL test. |
| 生物活性: |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
| 复溶: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.? |
| 贮存条件: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件: |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
| 研究配景: |
Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. |
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