| 产品名称: |
Recombinant Human IL-6 |
| 产品形貌: |
Recombinant Human Interleukin-6 is produced by our E.coli expression system and the target gene encoding Val30-Met212 is expressed. |
| 卵白编号: |
P05231 |
| 分子量: |
20.9 KDa |
| 表观分子量: |
20 KDa, reducing conditions |
| 产品纯度: |
Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.001 ng/?g (0.01 EU/?g) as determined by LAL test. |
| 生物活性: |
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 20-100 pg/ml. |
| 复溶: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.? |
| 贮存条件: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件: |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
| 研究配景: |
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. |
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