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β-Tubulin Mouse mAb

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EM1029

规格：	价钱：
50?L	￥1280.00
100?L	￥1980.00

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Overview

产品名称:	β-Tubulin Mouse mAb
反应性:	Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast
宿主:	Mouse
稀释比:	WB 1:5,000 IHC 1:200 IF 1:100-200
免疫原:	Synthetic Peptide
贮存条件:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid?repeated?freeze-thaw?cycles.
克隆性:	Monoclonal
抗体亚型:	IgG1
浓度:	1mg/mL
实测条带:	55kDa
基因ID:	203068
卵白序列:	P07437
细胞定位:	Cytoplasm,Cytoskeleton,Microtubule
研究配景:	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
IF analysis of Hela with EM1029(Left) and DAPI (Right) diluted at 1:100.

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