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小鼠磷酸化腺苷酸活化卵白激酶(p-AMPK)酶联免疫吸附检测试剂盒

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ELK9450

规格：	价钱：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

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Overview 文献(2)

产品名称:	Mouse p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
反应性:	Mouse
又名:	p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1
反应类型:	Sandwich
迅速度:	0.58 ng/mL
标准品:	100 ng/mL
检测规模:	1.57-100 ng/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.

scientific reports

l-Tartaric acid mitigates diabetic cardiac damage and is associated with changes in autophagy, apoptosis, and inflammatory markers

日期：2026-07-08 IF：4.8
Istanbul Universitesi Sosyoloji Dergisi-Istanbul University Journal of Sociology

Antidiabetic Effect of Hydroalcoholic Extract of Myrtus communis L. Fruit in a Type 2 Diabetes Mouse Model

日期：2024-07-25 IF：0.3

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.096	2.010
50.00	1.575	1.489
25.00	1.211	1.125
12.50	0.826	0.740
6.25	0.556	0.470
3.13	0.389	0.303
1.57	0.236	0.150
0.00	0.086	0.000

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细密度

板内细密度（测定内的细密度）：CV%<8%

三个已知浓度的样品划分在1个酶标板上测试20次，，，，以评估剖析板内细密度。。。。。

板间细密度（测定板间间细密度）：CV%<10%

三个已知浓度的样品划分在3个差别的酶标板上测试40次，，，，以评估剖析板间的细密度。。。。。

接纳率

划分往差别样本中添加已知浓度的p-AMPK，，，，做接纳率实验，，，，得出接纳率规模清静均接纳率

样本类型	接纳率规模	平均接纳率
血清(n=5)	83-95%	89%
EDTA 血浆(n=5)	89-103%	96%
Heparin 血浆(n=5)	87-99%	93%

线性

将添加有p-AMPK的样天职别稀释2倍，，，，4倍，，，，8倍，，，，16倍做接纳实验，，，，得出接纳率规模

样本类型	1:2	1:4	1:8	1:16
血清(n=5)	87-101%	95-102%	78-97%	85-96%
EDTA 血浆(n=5)	82-93%	86-95%	81-96%	79-91%
Heparin 血浆(n=5)	90-99%	85-92%	88-96%	87-98%

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