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小鼠核因子κB亚基p65亲和肽(NFκB p65)酶联免疫吸附检测试剂盒

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ELK9154

规格：	价钱：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

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Overview 文献(3)

产品名称:	Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
反应性:	Mouse
又名:	NF kappa B p65delta3; NFKB3
反应类型:	Sandwich
迅速度:	12.2 pg/mL
标准品:	2000 pg/mL
检测规模:	31.25-2000 pg/mL
样本类型:	serum, plasma, tissue homogenates
反应时长:	3.5h
研究领域:	Immunology
Uniprot ID:	Q04207
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

LIFE SCIENCES

Berberine attenuates epithelial mesenchymal transition in bleomycin-induced pulmonary fibrosis in mice via activating A2aR and mitigating the SDF-1/CXCR4 signaling

日期：2023-06-01 IF：6.78
Cancers

Cancer-Preventive Activity of Argemone mexicana Linn Leaves and Interpretation of Its Orchestral Effect on TNF-α and NF-kB Signaling Modulation in DMBA/TPA Induced Skin Cancer Mice Model

日期：2023-11-21 IF：5.2
Cancers

Cancer-Preventive Activity of Argemone mexicana Linn Leaves and Its Effect on TNF-α and NF-κB Signalling

日期：2023-11-21 IF：5.2

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.054	1.959
1000.00	1.527	1.432
500.00	1.251	1.156
250.00	0.834	0.739
125.00	0.453	0.358
62.50	0.326	0.231
31.25	0.187	0.092
0.00	0.095	0.000

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细密度

板内细密度（测定内的细密度）：CV%<8%

三个已知浓度的样品划分在1个酶标板上测试20次，，，，以评估剖析板内细密度。。

板间细密度（测定板间间细密度）：CV%<10%

三个已知浓度的样品划分在3个差别的酶标板上测试40次，，，，以评估剖析板间的细密度。。

接纳率

划分往差别样本中添加已知浓度的NFκB-p65，，，，做接纳率实验，，，，得出接纳率规模清静均接纳率

样本类型	接纳率规模	平均接纳率
血清(n=5)	90-107%	98%
EDTA 血浆(n=5)	93-107%	100%
Heparin 血浆(n=5)	85-97%	91%

线性

将添加有NFκB-p65的样天职别稀释2倍，，，，4倍，，，，8倍，，，，16倍做接纳实验，，，，得出接纳率规模

样本类型	1:2	1:4	1:8	1:16
血清(n=5)	86-94%	81-95%	88-97%	85-92%
EDTA 血浆(n=5)	83-96%	93-102%	86-94%	90-98%
Heparin 血浆(n=5)	92-106%	85-97%	96-105%	88-96%

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