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人钠/氢交流因子1(NHE1)酶联免疫吸附检测试剂盒

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ELK3886

规格：	价钱：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下载说明 ①

Overview

产品名称:	Human NHE1(Sodium/Hydrogen Exchanger 1) ELISA Kit
反应性:	Human
又名:	SLC9A1; APNH; Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger; Antiporter,Na+/H+,Amiloride Sensitive; Na(+)/H(+) antiporter, amiloride-sensitive
反应类型:	Sandwich
迅速度:	0.055 ng/mL
标准品:	10 ng/mL
检测规模:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;
Uniprot ID:	P19634
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NHE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NHE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NHE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.368	2.275
5.00	1.625	1.532
2.50	1.205	1.112
1.25	0.835	0.742
0.63	0.561	0.468
0.32	0.324	0.231
0.16	0.221	0.128
0.00	0.093	0.000

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细密度

板内细密度（测定内的细密度）：CV%<8%

三个已知浓度的样品划分在1个酶标板上测试20次，，以评估剖析板内细密度。。。。。

板间细密度（测定板间间细密度）：CV%<10%

三个已知浓度的样品划分在3个差别的酶标板上测试40次，，以评估剖析板间的细密度。。。。。

接纳率

划分往差别样本中添加已知浓度的NHE1，，做接纳率实验，，得出接纳率规模清静均接纳率

样本类型	接纳率规模	平均接纳率
血清(n=5)	89-105%	97%
EDTA 血浆(n=5)	81-95%	88%
Heparin 血浆(n=5)	86-99%	92%

线性

将添加有NHE1的样天职别稀释2倍，，4倍，，8倍，，16倍做接纳实验，，得出接纳率规模

样本类型	1:2	1:4	1:8	1:16
血清(n=5)	89-101%	85-96%	95-104%	82-90%
EDTA 血浆(n=5)	85-93%	87-96%	97-105%	95-102%
Heparin 血浆(n=5)	83-92%	96-102%	81-93%	79-91%

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