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¥1680.00/48T ¥2400.00/96T ¥10200.00/96T*5
| 48T | ¥1680.00 |
| 96T | ¥2400.00 |
| 96T*5 | ¥10200.00 |
| 产品名称: | Rat TNFa(Tumor Necrosis Factor Alpha) ELISA Kit |
| 反应性: | Rat |
| 又名: | TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2 |
| 反应类型: | Sandwich |
| 迅速度: | 6.1 pg/mL |
| 标准品: | 1000 pg/mL |
| 检测规模: | 15.63-1000 pg/mL |
| 样本类型: | 血清、血浆、组织匀浆、细胞裂解液、细胞作育上清液和其他生物体液 |
| 反应时长: | 3.5h |
| 研究领域: | 细胞因子;肿瘤免疫;熏染免疫学; |
| Uniprot ID: | P16599 |
| 检测原理: | 本试剂盒接纳夹心法原理。。。。。将特异性抗大鼠 TNFa抗体包被于96孔微孔板中,,,向微孔中划分加入大鼠 TNFa标准品或样本,,,使标准品中的大鼠 TNFa卵白或样本中的大鼠 TNFa卵白与固相于微孔板上的抗大鼠 TNFa抗体连系,,,然后加入生物素化抗大鼠 TNFa抗体,,,将未连系的生物素化抗体洗净后,,,加入HRP标记链亲和素,,,再次彻底洗涤后加入TMB底物显色。。。。。TMB在过氧化物酶催化下转化成蓝色,,,并在酸的作用下转化成最终的黄色。。。。。颜色的深浅和样品中的大鼠 TNFa卵白呈正相关。。。。。用酶标仪在450 nm波长下测定吸光度(OD值),,,通过绘制标准曲线盘算出样品浓度。。。。。 |
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Lycopene alleviates lipopolysaccharide-induced testicular injury in rats by activating the PPAR signaling pathway to integrate lipid metabolism and the inflammatory response
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Alpha-pinene neutralizes cisplatin-induced reproductive toxicity in male rats through activation of Nrf2 pathway
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Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway
Neuroprotective Role of THBS2 in Experimental Spinal Cord Injury Via its Anti-neuroinflammatory and Anti-apoptotic Properties
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Granisetron ameliorates doxorubicin-evoked nephrotoxicity via modulation of Nrf2 and TLR4/p38 MAPK/NLRP3 signals in rats
METTL14 knockdown attenuates neuron injury and improves function recovery after spinal cord injury via regulating FGF21 in a m6A-IGF2BP1 dependent mechanism
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| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.345 | 2.256 |
| 500.00 | 1.769 | 1.680 |
| 250.00 | 1.225 | 1.136 |
| 125.00 | 0.917 | 0.828 |
| 62.50 | 0.521 | 0.432 |
| 31.25 | 0.366 | 0.277 |
| 15.63 | 0.207 | 0.118 |
| 0.00 | 0.089 | 0.000 |

板内细密度(测定内的细密度):CV%<8%
三个已知浓度的样品划分在1个酶标板上测试20次,,,以评估剖析板内细密度。。。。。
板间细密度(测定板间间细密度):CV%<10%
三个已知浓度的样品划分在3个差别的酶标板上测试40次,,,以评估剖析板间的细密度。。。。。
| 样本类型 | 接纳率规模 | 平均接纳率 |
|---|---|---|
| 血清(n=5) | 80-90% | 85% |
| EDTA 血浆(n=5) | 84-106% | 100% |
| Heparin 血浆(n=5) | 87-93% | 90% |
| 样本类型 | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| 血清(n=5) | 89-97% | 85-94% | 87-96% | 85-97% |
| EDTA 血浆(n=5) | 85-94% | 87-96% | 92-101% | 82-98% |
| Heparin 血浆(n=5) | 85-99% | 98-106% | 86-99% | 78-95% |
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微信扫一扫连忙咨询
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