小鼠白介素1β(IL1b)酶联免疫吸附检测试剂盒-ELK意大利贵宾生物-ELISA试剂盒、抗体、卵白、分子

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小鼠白介素1β(IL1b)酶联免疫吸附检测试剂盒

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ELK1271

规格：	价钱：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

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Overview 文献(95)

产品名称:	Mouse IL1b(Interleukin 1 Beta) ELISA Kit
反应性:	Mouse
又名:	IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
反应类型:	Sandwich
迅速度:	6.5 pg/mL
标准品:	1000 pg/mL
检测规模:	15.63-1000 pg/mL
样本类型:	血清、血浆、组织匀浆、细胞裂解液、细胞作育上清液和其他生物体液
反应时长:	3.5h
研究领域:	细胞因子;肿瘤免疫;熏染免疫学;
Uniprot ID:	P10749
检测原理:	本试剂盒接纳夹心法原理。。。将特异性抗小鼠 IL1b抗体包被于96孔微孔板中，，向微孔中划分加入小鼠 IL1b标准品或样本，，使标准品中的小鼠 IL1b卵白或样本中的小鼠 IL1b卵白与固相于微孔板上的抗小鼠 IL1b抗体连系，，然后加入生物素化抗小鼠 IL1b抗体，，将未连系的生物素化抗体洗净后，，加入HRP标记链亲和素，，再次彻底洗涤后加入TMB底物显色。。。TMB在过氧化物酶催化下转化成蓝色，，并在酸的作用下转化成最终的黄色。。。颜色的深浅和样品中的小鼠 IL1b卵白呈正相关。。。用酶标仪在450 nm波长下测定吸光度(OD值)，，通过绘制标准曲线盘算出样品浓度。。。

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